Stem Cells International / 2014 / Article / Fig 3

Research Article

Reducing TRPC1 Expression through Liposome-Mediated siRNA Delivery Markedly Attenuates Hypoxia-Induced Pulmonary Arterial Hypertension in a Murine Model

Figure 3

Immunofluorescent (IF) staining for γ-H2AX+ cells in medial layer of pulmonary arterioles (PA) and lung parenchyma by day 28 after hypoxia-induced pulmonary arterial hypertension (PAH) (). ((a) to (c)) Identification of γ-H2AX+ cells in PA (white arrow) using IF double staining (i.e., α-SMA-γ-H2AX) in the three groups (400x). (d) versus other groups with different symbols (, †, ‡), . ((e) to (g)) Identification of γ-H2AX+ cells in lung parenchyma (white arrow) with IF double staining (i.e., α-SMA-γ-H2AX) in the three groups (400x). (h) versus other groups with different symbols (, †, ‡), . The scale bars in right lower corners represent 20 μm. Blue fluorescence indicates DAPI-stained nuclei. Statistical analysis in (d) and (h) using one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (, †, ‡) indicate significance (at 0.05 level).
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