Directing Parthenogenetic Stem Cells Differentiate into Adipocytes for Engineering Injectable Adipose Tissue

Figure 2

(a) Morphological appearance of pESC-derived EBs and outgrowths of EBs. (A) Micrograph of pESC-derived EBs. ((B)~(E)) Micrograph of outgrowths from 5 d EB plated on gelatin-coated plates for 2, 4, 6, 8, and 10 d. (F) Micrograph of eMSCs at passage 3 exhibiting spindle-shaped morphology (bars = 100 μm). (b) Schematic outlines of pESCs differentiation and adipose tissue engineering flow chart. (c) Transcription of specific markers for mesoderm, endoderm, and ectoderm differentiation. RNA samples from undifferentiated pESCs, EBs (5 days in suspension culture) and (+): subsequent adhesion culture days were analysed by RT-PCR. Nanog and OCT4 are ESCs markers. β-actin served as an internal standard for RT-PCR. M. DL2000 DNA marker.

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