Use of Autologous Mesenchymal Stem Cells Derived from Bone Marrow for the Treatment of Naturally Injured Spinal Cord in Dogs
Figure 2
Collection, culture, differentiation, and application of dog bone marrow mesenchymal stem cells (MSC), in dog 2. (a) and (b) control cell cultures in noninducing medium. (a) Day one of a first passage culture. (b) Cell confluence after 15 days of culture. (c) Adipogenic differentiation; Oil red staining showing cytoplasmatic fat granuli inside cells. (d) Osteogenic differentiation. Alizarin red staining showing mineralized matrix deposition within stem cells. Magnification: (a) and (b) 100X; (c) and (d) 400X.
