The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells
Figure 2
Cell densities for MAP-2 positive cells presenting neurites seven days after incubation with BMSCs and HUCPVCs CM. Immunocytochemistry assessment (using Cell-P software and 20x magnification micrographs) revealed that all CM tested conditions presented similar percentages of MAP-2 positive cells when compared to RA-differentiated cells, the positive control for SH-SY5Y differentiation (). The later effect was more noticeable for the BMSCs CM 24 h. Therefore, these results show that the secretome of both BMSCs and HUCPVCs is capable of inducing SH-SY5Y cells differentiation into neuronal-like cells (values are shown as mean ± SEM, , and statistical significance was defined as ).
