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Stem Cells International
Volume 2014, Article ID 438352, 10 pages
Research Article

The Secretome of Bone Marrow and Wharton Jelly Derived Mesenchymal Stem Cells Induces Differentiation and Neurite Outgrowth in SH-SY5Y Cells

1Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
2ICVS/3B’s, PT Government Associate Laboratory, Braga/Guimarães, Portugal

Received 11 February 2014; Revised 30 April 2014; Accepted 9 May 2014; Published 15 July 2014

Academic Editor: Pavla Jendelova

Copyright © 2014 Ana O. Pires et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary materials contain supplementary methods (cell metabolic viability, immunocytochemical, quantitative RT-PCR and neurite outgrowth assays), statistics, figures (representing the results obtained) and discussion regarding the use of low percentage of fetal bovine serum and retinoic acid as positive control of SH-SY5Y cells differentiation. Overall, results show that retinoic acid-differentiated cells not only provide a good positive control for proliferative cells differentiation, but are also a suitable control for SH-SY5Y cells differentiated with CM from both BMSCs and HUCPVCs.

  1. Supplementary Materials