Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells

Figure 3

Flow cytometric analysis of ROS production and mitochondrial membrane potential of MSCs. (a) DiD-labeled MSCs stained with DiOC6(3) (dash line) emitted quite similar fluorescent intensity compared to unlabeled MSCs (solid line) at 520 nm. (b) No significant differences in fluorescent intensity of DCFDA were also observed between DiD-labeled (dash line) and unlabeled MSCs (solid line). These data were expressed as mean ± SD of three independent experiments in triplicate.

(a)

(b)