Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells

Figure 4

MSCs inhibit proliferation and cytokine production by activated splenocytes. Unlabeled and DiD-labeled BALB/c-derived MSCs were added into 2-way MLR reaction of splenocytes (at ratio 1 : 10) and the proliferation of splenocytes (based on CFSE reduction) was checked after 5 days. Histogram of cell proliferation in MLR reaction in the absence of MSCs (a) and in the presence of unlabeled (b) or DiD-labeled MSCs (c) demonstrated suppression of splenocytes proliferation by MSCs, whether labeled with DiD or not. Unstimulated splenocytes showed the lowest level of CFSE dilution (d). Three days after initiation of MLR reaction, downregulation of IL-17, IFN-γ, and IL-10 and upregulation of PGE2 were observed in the culture supernatants of MLR reactions in the presence of unlabeled or DiD-labeled MSCs (e). NS: not significant; ; . These data were expressed as mean ± SD of three independent experiments in duplicate.

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