Effect of DiD Carbocyanine Dye Labeling on Immunoregulatory Function and Differentiation of Mice Mesenchymal Stem Cells

Figure 5

Therapeutic effects of allogenic MSCs in EAE mice. The mean ± SEM of clinical score of EAE in mice treated with three intraperitoneal injections of 1 × 10⁶ allogeneic MSCs with or without DiD as well as EAE controls is depicted in (a). MSCs with or without DiD labeling decrease the levels of IFN-γ and IL-17 and increase IL-10 levels in sera of treated mice compared to those in control EAE group (b). In vitro stimulation of splenocytes by MOG_35–55 peptide revealed no significant reduction in splenocytes proliferation in treatment groups compared to those of control group (c). , , and . These data were collected as mean ± SD from five mice in each group.

(a)

(b)

(c)