Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro
Figure 5
ALP activity. (a) CD90(−)/CD105(+) and unsorted ASCs contained higher protein concentrations. In contrast, CD90(−)/CD105(−) and BMSCs produced less protein in osteogenic conditions, even after BMP2 gene transfer. (b) ALP-positive staining (blue) appeared on day 7. CD90-positive ASCs and BMSCs (either with or without AdenoX-bmp2) showed an increased staining intensity. (c) The percentage of ALP positive area within the culture well was calculated. (d) Quantitative ALP activity was measured by normalizing total ALP amount to total protein. The results are expressed as means ± SD of three replicates. Statistical significance was identified as follows: , versus A (CD90(−)/CD105(−), AdenoX-bmp2(−)); #, versus A′ (CD90(−)/CD105(−), AdenoX-bmp2(+)); !, within each population; and $, versus the others excluding populations B′, D′ and F′ ().
