Stem Cells International

Research Article

Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro

Table 1

Summary of biological activities of the populations.
PopulationCD90(−)/CD105(−)CD90(+)/CD105(−)CD90(−)/CD105(+)CD90(+)/CD105(+)UnsortedBMSC
MediumNormalOsteogenicNormalOsteogenicNormalOsteogenicNormalOsteogenicNormalOsteogenicNormalOsteogenic
BMP2−−+−−+−−+−−+−−+−−+
ALP positive area ●● ●●●●● ●●● ●●●●● ●●●● 
(Data not shown)		●●●●●
Mineralized nodule ●● ●●●● ●●● ●●●● ●●●● ●●●●
Col 1        ●  ●● 
(Preculture)		  N/AN/AN/A
OC     ●  ●  ●   N/AN/AN/A
Each population of ASCs and BMSCs expressed different aspects in osteogenic characteristics after 14 days of culture. ●: detected; ●●: significantly increased to those in CD90(−)/CD105(−) cultured in the osteogenic medium without AdenoX-bmp2. ●●●: significantly increased to the others except ●●●. All cells cultured in DMEM without osteogenic supplements (normal) remained less osteogenic after the period.