Osteogenic Potential of Mouse Adipose-Derived Stem Cells Sorted for CD90 and CD105 In Vitro

Table 2

(a) CD90(+)/CD105(+) and BMSCs demonstrated strong adherent characteristics. In contrast, CD90(−)/CD105(−) exhibited weak adhesion. (b) The expression of homeobox and hedgehog signaling genes, which are related to body patterning and development, was relatively high in CD90(+)/CD105(−) cells. Strong expression of stem cell marker genes such as Nanog and Sox2 was also observed in CD90(+)/CD105(+). (c) Osteoblast-related genes were generally expressed at higher levels in CD90(+)/CD105(−) and CD90(+)/CD105(+) cells compared with CD90(−)/CD105(−) and unsorted ASCs. (d) Osteoclast-related signals were stronger in CD90(−)/CD105(−) and BMSCs. (e) A number ofWnt family genes were expressed at the highest levels in CD90(+)/CD105(+), followed by CD90(−)/CD105(+) and CD90(+)/CD105(−).

(a) Total expression levels of gene types related to cell adhesion

	Itga	Itgb	Cdh	Pcdh	Alcam/Vcam	Cadm	Lama,b
CD90(−)/CD105(−)	−16	−6	−26	−12	−3	1	−11
CD90(+)/CD105(−)	−1	3	−13	−6	−2	−8	7
CD90(−)/CD105(+)	−12	−5	−4	−6	−3	−2	−3
CD90(+)/CD105(+)	9	−4	12	7	−8	0	−12
Unsorted ASC	−13	−2	−30	−29	−5	−3	6
BMSC	7	1	6	−16	12	−2	−9

(b) Total expression levels of gene types related to cell proliferation

	Cell cycle			Body patterning and development								Oncogene and tumor suppressor							Stem cell marker
	stat	Creb	E2F	Shh	Pax	Hox	Fbx	Tbx	Dlx	Six	Fgf/Egf	Src	Rap	Asah	Bcl	Scrib	Ptpn	Tnf	Nanog	Sox2
CD90(−)/CD105(−)	−1	−3	−1	−3	2	−2	1	4	1	0	−3	0	0	−1	−2	0	−1	−3	−1	0
CD90(+)/CD105(−)	1	−1	−5	4	2	3	−5	−4	−2	−1	−1	−2	−1	1	1	−1	0	1	0	0
CD90(−)/CD105(+)	−3	−3	4	−2	2	3	−4	2	−1	−1	1	1	1	−1	−1	1	−1	−1	0	−3
CD90(+)/CD105(+)	−2	3	2	−2	−1	−2	−2	0	1	3	1	0	2	2	4	2	2	1	1	2
Unsorted ASC	1	−1	0	0	−2	0	−2	−2	−2	1	2	1	−1	0	−2	0	−1	−1	0	0
BMSC	0	−1	1	−3	1	0	1	−3	−5	−3	−3	−1	3	−3	−3	0	1	3	−1	−3

(c) Total expression levels of gene types related to osteoblast signaling

	Col1a	Bmp	Tgf/Tgfr	Fgf/Fgfr	Igf/Igfr	Pdgf/Pdgfr	smad	Wnt	Dcn	Vcan
CD90(−)/CD105(−)	1	−2	−7	0	−5	2	0	−2	−2	−3
CD90(+)/CD105(−)	1	8	−9	−2	−4	−3	0	−3	1	1
CD90(−)/CD105(+)	−3	−16	−1	−5	−4	1	2	−2	−1	−1
CD90(+)/CD105(+)	3	−7	−5	3	2	0	−1	0	2	1
Unsorted ASC	−3	−4	2	−1	−5	−6	1	0	−4	−1
BMSC	0	3	−4	2	−3	−1	−1	−2	0	−4

(d) Total expression levels of gene types related to osteoclast signaling

	Calcr	Ctsk	Sirpa	Csf	Tnf	Traf	Grb
CD90(−)/CD105(−)	−1	1	4	−2	−2	1	−1
CD90(+)/CD105(−)	−1	1	−4	0	1	−1	3
CD90(−)/CD105(+)	1	−1	0	1	−4	2	1
CD90(+)/CD105(+)	−2	−3	−2	−1	−1	1	−3
Unsorted ASC	1	−2	−5	0	1	−3	−2
BMSC	0	0	3	3	6	−1	2

(f) Total expression levels of gene isoforms related to Wnt signaling

Wnt

Fzd

Dv

Lrp

Sfrp

Dkk

Gsk3

Lef1

Nfat

Mapk

Smad

Sox

CD90(−)/CD105(−)

−14

−12

−2

3

−1

−2

0

0

−1

1

−6

2

CD90(+)/CD105(−)

−11

−4

−2

−2

0

3

2

0

−9

−4

1

0

CD90(−)/CD105(+)

−2

−2

−2

−1

−3

−6

2

3

10

−1

−3

−2

CD90(+)/CD105(+)

5

−2

−2

2

−1

5

−2

−2

−9

1

7

1

Unsorted ASC

−16

4

0

0

−1

−4

−3

−3

−2

1

−3

0

BMSC

−5

−3

2

−3

9

−6

0

−4

−4

−6

1

1

Wnt families

CD90(−)/CD105(−)

8b, 9b

CD90(+)/CD105(−)

2b, 3, 4, 5b, 6

CD90(−)/CD105(+)

2, 3a, 5, 5a, 9a, 9b

CD90(+)/CD105(+)

3a, 5a, 5b, 6, 9b, 10a, 10b, 11

Unsorted ASC

4

BMSC

4, 6, 7