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Stem Cells International
Volume 2014, Article ID 653734, 9 pages
http://dx.doi.org/10.1155/2014/653734
Research Article

Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells

1Department of Biology, Trinity Christian College, Palos Heights, IL 60463, USA
2Physician Assistant Studies/Allied Health Sciences, College of Health Professions, Grand Valley State University, Grand Rapids, IL 49503, USA

Received 30 June 2014; Revised 13 August 2014; Accepted 18 August 2014; Published 9 September 2014

Academic Editor: Gary E. Lyons

Copyright © 2014 Robert A. Boomsma and David L. Geenen. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1 cells after 4 hours which was partially reduced by oleamide. After 20 hours, 99% MSC gained calcein, unaffected by oleamide. Double-labeling HL-1 cells with calcein and the membrane dye DiO resulted in transfer of both calcein and DiO to MSC. When HL-1 cells were labeled with calcein and MSC with DiO, MSC gained calcein while HL-1 cells gained DiO. Very little fusion was observed since more than 90% Sca-1 positive MSC gained DiO from HL-1 cells while less than 9% gained gap junction impermeant CMFDA after 20 hours with no Sca-1 transfer to HL-1 cells. Time dependent transfer of membrane DiD was observed from HL-1 cells to MSC (100%) and vice versa (50%) after 20 hours with more limited transfer of CMFDA. These results demonstrate that MSC and HL-1 cells exchange membrane components which may account for some of the beneficial effect of MSC in the heart after myocardial infarction.