Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

Figure 1

The PDE5 inhibitors T0156, sildenafil, and zaprinast stimulated the proliferation of SVZ neural stem cells following a 6 h or 24 h exposure. Cells were treated with 1 μM T0156 (a), 1 μM, 10 μM and 50 μM sildenafil (b), or 10 μM and 50 μM zaprinast (c) for 6 h, and with 1 μM T0156 (d), 1 μM sildenafil (e), or 10 μM zaprinast (f) for 24 h. The incorporation of EdU was assessed by flow cytometry. Data are expressed as means ± SEM of at least 4 independent experiments. (a), (d), (e), and (f) two-tailed 𝑡-test, *𝑃<0.05 and **𝑃<0.01, significantly different from control and, (b) and (c) one-way ANOVA (Dunnett’s post-test), *𝑃<0.05 and **𝑃<0.01, significantly different from control.

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