Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

Figure 10

Activation of sGC stimulates proliferation of NSC, increases ERK1/2 phosphorylation, and decreases p27^Kip1 levels. Cell proliferation following treatment with 20 μM YC-1 (a) for 24 h was assessed by the incorporation of EdU and analyzed by flow cytometry. Levels of phospho-ERK1/2 following treatment with 20 μM YC-1 (b) or YC-1 plus 1 μM KT5823 (c) and p27^Kip1 levels following treatment with YC-1 (d) or YC-1 plus 1 μM U0126 (e) or KT5823 (f) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. (a), (b), and (d) two-tailed 𝑡-test, *𝑃<0.05 and **𝑃<0.01, significantly different from control and (c), (e), and (f) one-way ANOVA (Bonferroni’s post-test), ⁺𝑃<0.05, significantly different from YC-1.

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