Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

Figure 8

Inhibition of MAPK pathway prevented the decrease of p27^Kip1 levels mediated by T0156. p27^Kip1 levels following treatment with 1 μM U0126 and 1 μM T0156 (a), 1 μM sildenafil (b), or 10 μM zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 5 independent experiments. One-way ANOVA (Bonferroni’s post-test), *𝑃<0.05, significantly different from control and ⁺⁺𝑃<0.01, significantly different from T0156.

(a)  

(b)

(c)