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Stem Cells International
Volume 2014, Article ID 878397, 13 pages
Research Article

Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

1Centre for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal
2Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139 Faro, Portugal
3Institute for Biotechnology and Bioengineering (IBB), Centre for Molecular and Structural Biomedicine, University of Algarve, 8005-139 Faro, Portugal

Received 4 July 2013; Revised 20 November 2013; Accepted 24 November 2013; Published 12 January 2014

Academic Editor: Su-Chun Zhang

Copyright © 2014 Ana I. Santos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The expression of several PDEs normally found in the brain was analyzed in SVZ cultures by reverse-transcription PCR. We studied the presence of the cGMP-specific isoforms, PDE5A, PDE6C, and PDE9A. We also assessed the expression of PDE1 (isoforms PDE1A, 1B and 1C), and PDE10A, which hydrolyze both cAMP and cGMP, as well as the cAMP-specific PDE7 enzyme isoforms, PDE7A, and PDE7B, whose presence in the brain is well documented [34–36]. We observed the presence of PDE1, PDE5, PDE7 and PDE9 in SVZ cultures, while PDE10 is absent (see available online at: Supplementary Figure 1). Moreover, expression of PDE6 catalytic subunit was not detected in SVZ-derived NSC cultures, but it was detected in the retina.

  1. Supplementary Materials