Whole Genome Expression Profiling and Signal Pathway Screening of MSCs in Ankylosing Spondylitis

Figure 3

Verification of differentially expressed genes of MSCs in AS patients cultured in inflammatory environment. RNA was isolated from MSCs of HD () and AS patients () after MSCs were cultured in inflammatory environment mimicked by TNF-α (10 ng/mL) and IFN-γ (10 ng/mL). Comparison of the levels of differentially expressed genes in MSCs from both groups was based on quantitative real-time polymerase chain reaction (qRT-PCR). Beta-actin was used as an internal control. The results showed that when grown in inflammatory culture medium, 9 of 22 differentially expressed genes from whole genomic expression analysis in AS patients were truly differentially expressed. These genes include GNA12, DUSP1, MAPK11, RAC1, MAPK3, TRAF3, IFNAR1, IL6, and IFNA1. The symbol “*” represents .