Figure 2: Western blotting of collagen-I and osteocalcin. BMSCs stimulation by different TLR ligands affected secretion of collagen-I and osteocalcin. The amount of collagen-I and osteocalcin was assessed by Western blotting analysis. Compared with BMSCs untreated which were used as negative control, cells stimulated by TLR-3 ligands and TLR-4 ligands secreted more collagen-I and osteocalcin . The effect of TLR-4 ligands was stronger than TLR-3 ligands.