Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation
Radiation exposure promotes optimal chemotaxis of MSC. The percentage was calculated 15 days after radiation exposure and compared according to the irradiation configuration (abdominal irradiation (AI), represented in gray strips; total body irradiation (TBI) in red; and sham control group in gray). The histogram (a) represents the presence of the human beta-globin gene in organs outside the abdominal area such as the brain and heart. The histogram (b) represents the presence of the human gene in abdominal pelvic organs such as the liver and jejunum. Each group consisted in 10 animals. 15 days after AI, 100% of brain and 83% of hearts have human DNA. AI resulted in a mobilization of injected human stem cells on all the body via the vasculature system promoting communication into and between organs.