Stem Cells International / 2014 / Article / Tab 3

Research Article

Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation

Table 3

Levels of hMSC engraftment at 4 different time points after abdominal irradiation at 8.5 Gray.

Organs (positive per mice)Days after irradiation
3 days15 days30 days60 days

Brain4/10 (40%)10/10 (100%)2/6 (33%)1/10 (10%)
Heart2/10 (20%)5/10 (50%)4/6 (67%)4/10 (40%)
Lung7/10 (70%)10/10 (100%)4/6 (67%)2/10 (20%)
Liver1/10 (10%)10/10 (100%)5/6 (83%)0/10 (0%)
Spleen3/10 (30%)10/10 (100%)5/6 (83%)2/10 (20%)
Abdominal area
 Kidney3/10 (30%)10/10 (100%)4/6 (67%)2/10 (20%)
 Muscle1/10 (10%)2/10 (20%)6/6 (100%)1/6 (17%)
 Stomach1/10 (10%)9/10 (90%)5/6 (83%)0/8 (0%)
 Duodenum0/10 (0%)2/10 (20%)2/6 (33%)1/10 (10%)
 Jejunum1/10 (10%)10/10 (100%)4/6 (67%)1/10 (10%)
 Ileum1/10 (10%)1/10 (10%)2/6 (33%)1/10 (10%)
 Cecum0/10 (0%)1/10 (10%)1/6 (17%)1/10 (10%)
 Proximal colon0/10 (0%)1/10 (10%)2/6 (33%)3/10 (30%)
 Distal colon0/10 (0%)1/10 (10%)2/6 (33%)2/10 (20%)
Posterior leg (right)
 Skin0/10 (0%)1/10 (10%)0/6 (0%)0/10 (0%)
 Muscle 1/10 (10%)4/10 (40%)2/6 (33%)1/8 (12.5%)
 Bone 1/10 (10%)3/10 (30%)5/6 (83%)5/10 (50%)
 Bone marrow0/10 (0%)10/10 (100%)3/6 (50%)4/8 (50%)
Blood0/10 (0%)3/10 (30%)0/6 (0%)0/10 (0%)
Number of positive organs23/190102/19058/11431/186
Frequency12%54%51%17%

The biodistribution of human cells is maximum at 15 days. hMSC engraftment is detectable from 3 days after irradiation to at least 60 days. hMSC engraftment varies from 12% of organs at day 3, 54% on D15, 51% on D30, to 17% at day 60. The homing of human cells appeared to increase up to day 15 and then decreased with time.