Stem Cells International / 2014 / Article / Tab 4

Research Article

Long-Term Quantitative Biodistribution and Side Effects of Human Mesenchymal Stem Cells (hMSCs) Engraftment in NOD/SCID Mice following Irradiation

Table 4

Kinetics of hMSC biodistribution after total body irradiation (TBI) at 3.5 Gray.

Organs (positive per mice)Days after irradiation
3 days7 days15 days30 days60 days90 days120 days

Brain0/10 (0%)0/10 (0%)6/8 (75%)2/6 (33%)4/8 (50%)0/8 (0%)0/6 (0%)
Heart8/10 (80%)6/8 (75%)7/8 (88%)6/8 (75%)0/8 (0%)4/8 (50%)4/6 (67%)
Lung4/10 (40%)10/10 (100%)8/8 (100%)6/8 (75%)0/8 (0%)3/8 (38%)6/6 (100%)
Liver2/10 (20%)0/10 (0%)8/8 (100%)4/7 (57%)4/8 (50%)0/8 (0%)0/6 (0%)
Spleen0/10 (0%)6/8 (75%)5/8 (63%)4/7 (57%)4/8 (50%)2/6 (33%)0/6 (0%)
Kidney0/10 (0%)0/10 (0%)4/8 (50%)3/7 (43%)0/8 (0%)0/8 (0%)2/6 (33%)
Abdominal area
 Muscle2/10 (20%)5/10 (50%)2/6 (33%)0/8 (0%)0/6 (0%)4/8 (50%)2/6 (33%)
 Stomach4/10 (40%)6/8 (75%)4/7 (57%)4/6 (67%)0/8 (0%)3/8 (38%)1/6 (17%)
 Duodenum2/10 (20%)5/10 (50%)2/6 (33%)0/7 (0%)0/8 (0%)0/8 (0%)0/6 (0%)
 Jejunum2/10 (20%)0/10 (0%)2/8 (25%)2/8 (25%)0/8 (0%)2/8 (25%)0/6 (0%)
 Ileum0/10 (0%)0/10 (0%)0/8 (0%)0/8 (0%)0/8 (0%)0/8 (0%)0/6 (0%)
 Cecum0/10 (0%)0/10 (0%)3/8 (38%)3/8 (38%)0/8 (0%)2/8 (25%)2/6 (33%)
 Proximal colon0/10 (0%)0/10 (0%)3/8 (38%)3/7 (43%)0/8 (0%)2/8 (25%)0/6 (0%)
 Distal colon2/10 (20%)2/8 (25%)0/8 (0%)1/5 (20%)0/8 (0%)0/8 (0%)2/6 (33%)
Posterior leg (right)
 Skin0/10 (0%)0/10 (0%)3/8 (38%)0/6 (0%)0/8 (0%)3/8 (38%)0/6 (0%)
 Muscle 0/10 (0%)5/10 (50%)7/8 (88%)2/8 (25%)4/8 (50%)0/8 (0%)0/6 (0%)
 Bone 0/10 (0%)2/8 (25%)1/8 (12.5%)0/8 (0%)4/8 (50%)2/8 (25%)0/6 (0%)
 Bone marrow0/10 (0%)1/8 (12.5%)5/8 (63%)3/8 (38%)0/8 (0%)0/8 (0%)1/6 (17%)
Blood0/10 (0%)0/10 (0%)1/8 (12.5%)4/7 (57%)0/8 (0%)0/8 (0%)0/6 (0%)
Number of positive organs26/190 48/17871/15147/13740/15027/15020/114
Frequency14%28%47%34%27%18%18%

The time at which the implantation of hMSCs is optimum varies from one organ to another. hMSCs were detectable at least up to 120 days after injection of human cells in a number of organs. Abdominal irradiation as compared to TBI modifies hMSC engraftment in the exposed area. Abdominal irradiation increased hMSC engraftment from 3 to 60 days in the stomach, duodenum, jejunum, and ileum and decreased engraftment in cecum and colon.