Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells

Figure 2

T-MSCs inhibit differentiation and maturation of DCs by secretion of soluble factors. (a) BMCs and T-MSCs were cultured in a transwell plate and BMCs were induced to differentiate into DCs by GM-CSF. (b) BMCs differentiation into CD11b⁺ cells was inhibited by T-MSCs added at day 0 (). (c) CD86 expression on mature DCs was significantly downregulated by T-MSCs (). Similar to the coculture condition, MHC class II expression on immature and mature DCs was inhibited by factors secreted from T-MSCs (). CD14 expression by T-MSCs showed a trend toward increase, but it was not significant.