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Stem Cells International
Volume 2015, Article ID 106540, 12 pages
http://dx.doi.org/10.1155/2015/106540
Research Article

Immune Suppressive Effects of Tonsil-Derived Mesenchymal Stem Cells on Mouse Bone-Marrow-Derived Dendritic Cells

1Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea
2Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 158-710, Republic of Korea

Received 21 November 2014; Revised 9 January 2015; Accepted 24 January 2015

Academic Editor: Armand Keating

Copyright © 2015 Minhwa Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

For the comparison of T-MSCs with BM-MSCs, we performed the phenotype analysis by flow cytometer and it shows that both MSCs lack of CD14, CD34, and CD45 but express CD73, CD90, and CD105 (Supplement Figure 1). Therefore, we concluded that T-MSCs, we had isolated and used in this study, show similar phenotype to that of BM-MSCs. In order to analyze the immune suppressive effect of T-MSCs on DC differentiation and maturation, we performed co-culture assay with dose response experiments in supplement Figure 2. It shows that even under 100:1 (mature DC to T-MSC ratio) decrease of CD11b, CD80, CD86 and class II MHC molecules are statistically different on DCs.

  1. Supplementary Material