Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

Figure 2

SVF recovery by filtration. (a) The figure shows a representative flow cytometry light scatter profile for SVF cells that have been recovered from the retentate and permeate fractions of filters of indicated pore size in μm. Percentage values represent the proportion of cells retained on the filter membrane relative to the total cell number in the whole SVF before filtration. Data shows a representative set of light scatter plots from four individual donors in each group. Events in gates P1 (red) and P2 (green) represent the and populations, respectively. (b) Antibody labelling and flow cytometric detection of cell surface markers expressed by SVF cells recovered by centrifugation or filtration using 5 μm PCTE filter. The percentage positive cells for each marker combination is calculated after subtraction of the nonspecific fluorescence obtained with the isotype controls. Data shows a representative set of plots from four individual donors in each group. Plots represent events from both (red) and (green) populations.

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