Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

Table 2

SVF yield from lipoaspirates processed by whole digest centrifugation and aqueous phase separation.

Volume of fat	Centrifugation yield	Phase separation yield
(mL)	(×10^5/gram)	(×10^5/gram)
50 mL	2.05 ± 0.7 (100%)	2.00 ± 0.4 (101 ± 17%)
100 mL	1.45 ± 0.8 (100%)	1.48 ± 0.6 (99 ± 08%)
200 mL	1.00 ± 0.2 (100%)	0.89 ± 0.1 (87 ± 27%)
500 mL	1.32 ± 0.6 (100%)	1.6 ± 1.0 (117 ± 18%)

Cell yield is represented as mean cell number per gram of adipose tissue from three to seven donor samples processed for each volume. Percentage values indicate percentage recovery by aqueous phase separation as relative to centrifugation of the whole tissue digest. Data is represented as mean ± SD.