Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Plating density</td><td align="center" rowspan="2">Centrifugation</td><td align="center" rowspan="2">Phase separation</td></tr><tr class="thead"><td align="left">(SVF cells/10 cm<sup>2</sup>)</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">20000</td><td align="center">72 ± 16</td><td align="center">101 ± 24</td></tr><tr><td align="left">10000</td><td align="center">48 ± 14</td><td align="center">42 ± 16</td></tr><tr><td align="left">5000</td><td align="center">20 ± 6</td><td align="center">21 ± 5</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr><tr class="table-fn"><td>Data represents mean number of CFU-F obtained for each plating density from three individual donors, each assayed in duplicate. Errors represent SD from three individual donor samples.<br/></td></tr></table>

Fibroblastic colony forming unit (CFU-F) assay for SVF recovered by aqueous phase separation as compared to whole digest centrifugation.

Stem Cells International

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Table 3

Table 3: Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate 