Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

Table 4

Yield and viability of SVF obtained by the automated process using filtration technology as compared to the manual process of cell isolation using centrifugation.

	Digestion		Filtration
	Manual	Automated	Manual	Automated
SVF yield (×10^5/gram)	0.90 ± 0.5	0.82 ± 0.6	1.15 ± 0.3	1.17 ± 0.5
Percentage yield	100%	94 ± 28%	100%	98 ± 21%
Percentage viability	96 ± 3.0%	96 ± 2.1%	97.3 ± 1.5%	97.5 ± 2.8%

Data is represented as mean ± SD for five individual samples tested. SVF Yield is represented as cell number ×10⁵/gram of adipose tissue. Percentage yield indicates percentage recovery by automated processing relative to the yield from manual processing taken as the baseline comparator. Difference in cell yield between manual centrifugation method and automated filtration is not significant (, paired -test).