Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate

Table 5

Composition of SVF isolated by the automated process as compared to manual isolation.

Cell type	Marker profile	Manual	Automated
ASC	CD34^+CD31^−	20 ± 5	25 ± 9
EPC	CD34^+CD31^+	30 ± 10	24 ± 6
Mature EC	CD34^−CD31^+	4 ± 3	5 ± 1
Pericyte	CD31^−CD146^+	1.9 ± 1.2	2.0 ± 1.4
Lymphocytes	HLA DR^+	24 ± 10	27 ± 10
RBC	GpA^+	12 ± 5	15 ± 11

Table represents mean percentage positive cells with standard error, from four different data sets. ASC: adipose derived stem/stromal cells; EPC: endothelial progenitor cells; RBC: red blood cells.