Stem Cells International

Review Article

The Role of Adipose-Derived Stem Cells in Breast Cancer Progression and Metastasis

Table 3

Relevant in vivo studies investigating the effects of ADSCs on breast cancer.


Reference	Year	Model	ADSC origin	ADSC surface markers	BCC line	Ratio BCC/ADSC	Effects on BCC/BC

Eterno et al. [60]	2014	Mouse	Human lipoaspirates and breast whole fat (normal versus cancer-affected)	CD44⁺CD90⁺CD117⁺ CD133⁺ CD34^lowCD45⁻	MCF-7, MDA-MB-231, primary BCCs	2 : 1	No changes in MCF-7 MDA-MB-231 growth and migration↑ EMT↑ Paracrine, IL-8↑, IL-6↓

Rowan et al. [71]	2014	Mouse	Human lipoaspirates (abdominal)	CD29⁺CD34⁺CD73⁺ CD90⁺CD105⁺ CD44^lowCD45^low	BT-474, MCF-7, MDA-MB-231	1 : 1	Tumor growth↔ Migration and metastasis↑ EMT induction

Orecchioni et al. [41]	2013	Mouse	Human lipoaspirates	CD31⁺CD34⁺CCRL2+ CD13⁻CD45⁻ (EPC) and CD13⁺CD34⁺ CD140b⁺ CD31⁻CD45⁻ (ADSC)	HCC1937, MDA-MB-436, ZR75-1	5 : 1	Tumor growth↑ Metastatic spread↑ EMT↑ Effect of ADSCs > EPCs

Chandler et al. [36]	2012	Mouse	Human lipoaspirates	CD13⁺CD29⁺CD44⁺ CD73⁺CD90⁺CD105⁺ CD166⁺ CD14⁻CD31⁻CD45⁻	MCF-7, MDA-MB-231	1 : 1	Tumor growth↑ Angiogenesis↑ Bidirectional signaling ADSCs differentiate to CAFs

Zhang et al. [22]	2012	Mouse	Murine (endogenous)	CD34⁺ CD31⁻CD45⁻	E0771, MDA-MB-231	n.s.	Circulating ADSCs↑ in cancer ADSCs incorporate into tumor vasculature (as pericytes)

Zhao et al. [74]	2012	Mouse	Human breast (normal)	CD29⁺CD73⁺CD90⁺ CD105⁺ CD14⁻CD31⁻CD45⁻	HMT-3522 S3 (preinvasive), HMT-3522 T4-2 (invasive), MDA-MB-231	1 : 1, 3 : 2	Tumor growth↑ Tumor invasiveness↑ Angiogenesis↔ No effect on preinvasive BCCs

Dirat et al. [39]	2011	Mouse	Murine 3T3 adipocytes	—	4T1, 67NR, (murine) ZR 75.1, SUM159PT	n.s.	Metastatic spread↑ IL-6-dependent

Martin-Padura et al. [43]	2012	Mouse	Murine whole fat	CD34⁺ CD45⁻	HCC1937, MDA-MB-436	5 : 1	Tumor growth↑ Metastatic spread↑ Angiogenesis↑

Zimmerlin et al. [5]	2011	Mouse	Human abdominal whole fat	CD34⁺CD44⁺CD73⁺ CD90⁺CD105⁺CD146⁺ CD45⁻CD31⁻	Human MPE	n.s.	Tumor growth↑ (active cells, but not resting cells)

Muehlberg et al. [30]	2009	Mouse	Murine whole fat	CD44⁺CD90⁺CD105⁺ CD11b⁻CD14⁻CD34⁻ CD45⁻HLA-DR⁻	4T1 (murine), MDA-MB-231	1 : 10	Tumor growth↑ Metastatic spread↑ Paracrine through SDF-1 ADSCs home to tumor and differentiate to ECs

Sun et al. [49]	2009	Mouse	Human breast whole fat	n.s.	MDA-MB-231	2 : 1	Tumor growth↓ Metastatic spread↓ No early carcinogenesis improvement

Walter et al. [42]	2009	Mouse	Human breast whole fat and abdominal lipoaspirates	n.s.	MCF-7, MDA-MB-231	1 : 1	Tumor migration and invasiveness↑ IL-6-dependent

Zhang et al. [89]	2009	Mouse	Murine whole fat (obese mice)	CD34⁺ CD31⁻CD45⁻ (ADSC) and CD31⁺CD34⁺ CD45⁻ (EPC)	4T1, EF43.fgf4 (murine), MDA-MB-231	n.s.	Tumor growth↑ ADSCs home to tumor (perivascular space)

ADSC: adipose-derived stem cell; BC: breast cancer; BCC: breast cancer cell; EC: endothelial cell; EMT: epithelial-to-mesenchymal transition; EPC: endothelial progenitor cell; ER: estrogen receptor; Her2: human epidermal growth factor receptor 2; MMPs: matrix metalloproteinases; MPE: metastatic pleural effusion; n.s.: not specified; PR: progesterone receptor; T reg: regulatory T lymphocyte.