Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis

Figure 5

MSCs injections at different stages of EAE diminished brain T-bet, RORγT, and Foxp3 mRNA levels. Healthy C57BL/6, EAE mice, or EAE mice injected with MSCs (1 × 10⁶) at the onset (), at the peak (), and at the time of EAE stabilization () were sacrificed after 50 days of EAE progression. Total brain RNA was extracted (Trizol) and 2 μg of DNAse-treated RNA was retrotranscribed as described in Methods. Relative mRNA levels of bona fide transcriptions factors associated with CD4+ T helper subtypes Th1 (T-bet, (a)), Th17 (RORγT, (b)), and Treg (Foxp3, (c)) were determined by RT-qPCR using the method. Individual ratios, (d) T-bet/RORγT, (e) Foxp3/T-bet, and (f) Foxp3/RORγT were also calculated. Bars in the plots represent the mean ± SEM. Statistical differences were calculated using Mann-Whitney test (, ), .

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