Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis

Figure 7

MSCs administration significantly diminished IL-6 plasma levels independently of the time of injection. Plasma samples were isolated from EAE mice, treated or not (EAE) with MSCs at different time of EAE progression, at the onset (), at the peak (), and at the time of EAE stabilization (). Blood samples were taken (day 0), at the onset (day +10), before the peak (day +16), after the peak (day +22), at the time of EAE stabilization (day +30), after EAE stabilization (day +36), and at the end of experiment (day +50). Plasma samples were isolated by centrifugation and stored at −80°C until being used. Cytokine levels IL-6 (a), TNF-α (b), IL-17F (c), and TNF-β (d) were detected using Milliplex mouse Th17 magnetic bead panel on Luminex 200 (Merck, Millipore). The samples of each EAE group () were pooled and analyzed in triplicate. Bars represent the mean ± SEM; statistical differences were calculated using Mann-Whitney test ().

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