Hematopoietic Origin of Murine Lung Fibroblasts

Figure 2

Transplantation of nonadherent bone marrow results in multilineage hematopoietic engraftment. Shown is a representative analysis of total (a) and multilineage (b) engraftment approximately 2 months after transplantation of nonadherent bone marrow cells. Isotype (top panels) forward scatter and side scatter gate is shown (left) with IgG-PE versus EGFP gate (right). Representative total engraftment is shown in Panel (a) (bottom panels) with forward scatter and side scatter gate (left) and PE and EGFP gates shown (right). (b) Representative multilineage engraftment analysis is depicted in Panel (b). The isotype IgG PE gate and the EGFP population gated on the IgG PE population are depicted in (i). Analysis of B cell (ii), T cell (iii), and granulocyte/macrophage (iv) populations demonstrates EGFP⁺ cells representing 82.5%, 36.5%, and 85.9% of each lineage, respectively. In all dot plots (i–iv), total populations are shown on the left, and EGFP⁺ cells within the gated population are shown on the right. See Table 1 for engraftment for all mice.

(a)  

(b)