Hematopoietic Origin of Murine Lung Fibroblasts

Figure 3

HSC-derived circulating fibroblast precursors are present in the peripheral blood of engrafted mice. Peripheral blood of engrafted nonadherent bone marrow transplanted mice was analyzed for the presence of CFPs. Representative forward scatter and side scatter gate is shown Panel (a) and isotype control is shown in Panel (b). Panels (c)–(e) depict analysis of peripheral blood from a representative animal. Forward and side scatter gate is shown in Panel (c). The population expressing CD45 (PE) and DDR2 (APC) is shown in Panel (d) (upper right quadrant). Analysis of EGFP expression of gated CD45⁺/DDR2⁺ population from Panel (d) is shown in Panel (e) (94.6%).

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