Hematopoietic Origin of Murine Lung Fibroblasts

Figure 5

EGFP⁺ lung fibroblasts are present in the lungs of nonadherent transplant engrafted mice. Differential interference contrast (DIC), Hoechst nuclear stain (HO), GFP, Collagen I, and DDR2 antibody stain are shown from a representative lung section from an engrafted animal in Panels (a)–(e), respectively. Panel (f) shows merged image of GFP (green), Collagen I (red), and DDR2 (blue) staining. A representative GFP⁺/Collagen I⁺/DDR2⁺ cell is indicated by *; a representative GFP⁺/Collagen I⁺ cell is indicated by an open arrow head; a representative GFP⁺/DDR2⁺ cell is indicated by a solid arrow head. Panel (g) shows higher magnification of inset (boxed area) in Panel (f) with * indicating a GFP⁺/CD45⁺/DDR2⁺ expressing cell. Panels (h)–(n) depict images of secondary only controls. Bar = 25 μM.