Hematopoietic Origin of Murine Lung Fibroblasts

Figure 6

Hematopoietic stem cells give rise to lung fibroblasts in vivo. Differential interference contrast (DIC), Hoechst nuclear stain (HO), EGFP, Collagen I, and DDR2 antibody stain are shown in Panels (a)–(e), respectively, from a representative section of lung tissue from a clonally engrafted mouse. Panel (f) shows merged image of GFP (green), Collagen I (red), and DDR2 (blue) staining. Panel (g) shows higher magnification of inset (boxed area) in Panel (f) with * indicating a GFP/Collagen I/DDR2 expressing cell. Panels (h)–(n) depict images from secondary only staining controls.