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Stem Cells International
Volume 2015, Article ID 159713, 11 pages
Research Article

Hematopoietic Origin of Murine Lung Fibroblasts

1Research Services, Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston, SC 29401, USA
2Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29425, USA
3Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA

Received 14 February 2015; Revised 21 May 2015; Accepted 25 May 2015

Academic Editor: Renke Li

Copyright © 2015 Lindsay T. McDonald et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

HSC-derived lung fibroblasts are not an artifact of irradiation. Differential interference contrast (DIC), Hoechst nuclear stain (HO), CD45, DDR2 antibody stain are shown in Panels A–D, respectively, from a representative section of lung tissue from a non-irradiated mouse. Panel E shows merged image of HO (blue), CD45 (green), DDR2 (red) staining. Panel F shows higher magnification of inset (boxed area) in Panel E with * indicating a CD45/DDR2 expressing cell (green and red). Panels G–L depict images from secondary only staining controls. 400x magnification, mag bar = 25 μM

  1. Supplementary Material