Acute Lymphoblastic Leukemia Cells Inhibit the Differentiation of Bone Mesenchymal Stem Cells into Osteoblasts In Vitro by Activating Notch Signaling

Figure 2

ALL cells activate Notch signaling in BMSCs in coculture. (a), (c) The Jagged1 expression levels in ALL cells and normal BMNCs were evaluated by real-time RT-PCR and western blotting ( versus BMNCs). (b), (d) The mRNA and protein expression levels of Notch1 and Hes1 in BMSCs were analyzed using real-time RT-PCR and western blot analysis after 3-day cocultured with ALL cells or BMNCs ( versus BMSCs cultured alone or coculture with BMNCs).

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