Acute Lymphoblastic Leukemia Cells Inhibit the Differentiation of Bone Mesenchymal Stem Cells into Osteoblasts In Vitro by Activating Notch Signaling

Figure 4

Effect of anti-Jagged1 neutralizing Ab on osteoblast differentiation of BMSCs in cocultures. BMSCs2 and ALL cells were cocultured with or without anti-Jagged1 neutralizing Ab under osteogenic conditions for 3 days or 14 days. (a-b) The mRNA and protein expression levels of osteogenic differentiation markers OPN and OCN, and Notch1 and Hes1 were analyzed using real-time RT-PCR and western blot analysis after 3-day coculture. (c) ALP levels were detected using an ALP kit ( versus BMSCs2 cocultured with ALL cells). (d) Calcium deposits were detected using von Kossa staining.

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