Differential Regulation of Gene Expression of Alveolar Epithelial Cell Markers in Human Lung Adenocarcinoma-Derived A549 Clones

Figure 5

Effects of DCIK treatment following U0126 treatment. (a) Experimental design. B7 and H12 were treated with DCIK for 3 days following U0126 30 µM treatment for 24 h. After DCIK removal, B7 and H12 were cultured with serum or without serum for 3 days. (b) Morphological change of B7 treated without DCIK (control) and with DCIK (DCIK) at every 24 h for 3 days following U0126 treatment for 24 h. Scale bar: 100 µm. (c) Expression and quantitative analysis of SPC, AQP5, TTF-1, and SPB mRNAs in B7. (Upper panel) RT-PCR results. (Lower four panels) quantitative analyses of gene expression. Black: cultured with serum, red: cultured without serum, green: cultured without DCIK following U0126 treatment for 24 h, light blue: cultured with DCIK following U0126 treatment for 24 h and cultured with serum following DCIK treatment, and purple: cultured without serum following DCIK treatment. (d) Morphological change of H12 treated without DCIK (control) and with DCIK (DCIK) at every 24 h for 3 days following U0126 treatment for 24 h. Scale bar: 100 µm. (e) Expression and quantitative analysis of SPC, AQP5, TTF-1, and SPB mRNAs in H12. (Upper panel) RT-PCR results. (Lower four panels) quantitative analyses of gene expression. Indications were the same as in (c). All data were independently obtained twice.