Octanoate in Human Albumin Preparations Is Detrimental to Mesenchymal Stromal Cell Culture

Figure 2

Growth and colony forming ability of donor MSCs. Pooled results represent the averages of three separate donors. Donor MSCs were grown in PPRF-msc6 with various albumins or in DMEM 10% FCS for two passages before growth analysis in a 96-well plate based assay. All population doubling times were significantly different to each other () and were calculated from cell numbers at 24 and 72 hours (a). Colony forming ability was measured following similar growth conditions for either 2 or 4 consecutive passages (b). Results are combined from 3 separate donors and experiments repeated at least twice. Error bars represent standard error of the mean, ** indicates a value of <0.01, and * indicates a value of <0.05.