Modifications of Human Subcutaneous ADMSC after PPARγ Activation and Cold Exposition

Figure 4

Effect of IL4 in the levels of adipocytokines. (a) The differentiated adipocytes were obtained and treated as describe before; total protein was isolated for detecting expression of adiponectin and PGC-1α by western blot. IL4 was added to cells during a period of 4 hours and at the same time cells were exposed to 37°C or 31°C. (b) Relative intensity levels were determined by densitometry of bands. Analyses were performed using ANOVA test. Data are expressed as mean ± SD and differences were considered significant with . The differences between the cells treated with IL4.

(a)

(b)