Review Article
Cell Cycle-Driven Heterogeneity: On the Road to Demystifying the Transitions between “Poised” and “Restricted” Pluripotent Cell States
Figure 2
Fucci hESCs can be used to isolate cell cycle fractions from live cells. (a) Image of live Fucci hESCs. Bar: 25 μm. (b) Flow cytometric analysis of Fucci hESCs, showing early-G1, EG1; late-G1, LG1; S phase, S; and G2/M-phases, G2/M. (c) Diagram showing that transcripts expressed from developmental genes have been found to be cell cycle regulated, peaking in G1 and downregulated in S phase.
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