Morphology of MSCs and cell surface antigen expression. BMSCs, AMSCs, and SMSCs were cultured in separate 35 mm dishes and displayed the morphology at 4 passages in phase contrast images (a). Three kinds of MSCs were analyzed for MSCs specific surface antigen expression by flow cytometry (b). Open histograms imply isotype IgG expression as control, and filled histograms represent each surface antigen expression. Mean% ± SD of each MSCs expression in respective three biological replications was presented on top of each graph. BMSCs: bone marrow derived mesenchymal stem cells; AMSCs: adipose derived mesenchymal stem cells; SMSCs: skin derived mesenchymal stem cells. Magnification ×100.