Confirmation of differentiation ability into various mesenchymal lineages. Three kinds of MSCs were differentiated into adipocytes, osteocytes, and chondrocytes for three weeks. (a) Cytochemical staining of each differentiated MSCs with Oil red O, Von Kossa, AP staining, and alcian blue. (b) Increased intensities of lineage specific gene expression before and after differentiation in each MSCs by RT-PCR. Lanes, which were displayed from the left to the right, showed 100 bp or 200 bp ladder, undifferentiated MSCs, and differentiated cells in numerical order of BMSCs, AMSCs, and SMSCs, respectively. AP2: adipocyte fatty acid binding protein; LPL: lipoprotein lipase; OPN: osteopontin; ON: osteonectin; ACAN: aggrecan; COL10A1: collagen, type X, alpha 1; BMSC-A, BMSC-O, and BMSC-C: differentiated BMSC into adipocytes, osteoblasts, and chondrocytes, respectively; AMSC-A, AMSC-O, and AMSC-C: differentiated AMSC into adipocytes, osteoblasts, and chondrocytes, respectively; SMSC-A, SMSC-O, and SMSC-C: differentiated SMSC into adipocytes, osteoblasts, and chondrocytes, respectively. Magnification ×100.