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Stem Cells International
Volume 2015, Article ID 247091, 12 pages
Research Article

Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro

1Department of Cardiothoracic Surgery, Heart Center, University of Cologne, 50937 Cologne, Germany
2Center for Molecular Medicine Cologne, University of Cologne, 50931 Cologne, Germany
3Institute for Neurophysiology, University of Cologne, 50931 Cologne, Germany
4Berlin-Brandenburg Center for Regenerative Therapies, 13353 Berlin, Germany
5Department of Anesthesiology, Perioperative and Pain Medicine, Children’s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA

Received 9 March 2015; Revised 27 June 2015; Accepted 2 July 2015

Academic Editor: Silvia Brunelli

Copyright © 2015 Klaus Neef et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material provided online contains representative images of immunocytochemical stainings for quantitative assessment of expression of skeletal muscle markers (S1) and cardiac markers (S2), relevant positive controls for antibody specificity (S3-S7), qPCR gene expression data for statistical analyses (S8) and representative images from electrophysiological analyses (S9).

  1. Supplementary Material