Protective Effect of Neuropeptide Substance P on Bone Marrow Mesenchymal Stem Cells against Apoptosis Induced by Serum Deprivation

Figure 3

Effect of SP on Wnt-related molecules in BMSCs. The mRNA and protein levels of the Wnt pathway proteins β-catenin, GSK-3β/p-GSK-3β, cyclin D1, and c-myc were evaluated by qPCR and Western blot analysis in BMSCs. A significant activating effect of SP on Wnt signaling was observed after 12 h (a). Various concentrations of SP (10⁻⁸, 10⁻¹⁰, and 10⁻¹² mol/L) upregulated the protein levels of Wnt pathway proteins (b). An NK-1 antagonist and the Wnt pathway inhibitor DKK1 both attenuated the activating effect of SP on Wnt signaling, indicating that the Wnt pathway mediates the effect of SP (c).

(a)

(b)

(c)