Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats

Figure 2

The BMSCs supernatant could promote the proliferation, migration, and repair of human umbilical-vein endothelial cell line (EA.hy926 cells). (a) The methylene blue staining (magnification, ×200) is indicating that the BMSCs supernatant could significantly promote the migration of EA.hy926 cells. Data are expressed as mean ± SD ( for DMEM-LG versus DMEM-LG + BMSCs supernatant). (b) The significant increase of the growth of EA.hy926 cells was investigated in MTT test after being given BMSCs supernatant. (c) Impaired EA.hy926 cells could be repaired by BMSCs supernatant. Data are expressed as mean ± SD (^# for sham versus normal, and for DMEM-LG versus DMEM-LG + BMSCs supernatant at each corresponding time point). Data analysis was performed by a one-way ANOVA. (BMSCs, bone marrow-derived mesenchymal stem cells, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,S-diphenyltetrazolium bromide).

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