Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1α/CXCR4 Axis in Rats

Figure 7

qRT-PCR or western-blot assays are showing that the expressions of ANG-1, VEGF, HGF, TGF-β, and CD31 in pancreatic tissue were significantly higher in SAP+BMSCs group. ((a), (c), and (d)) The expression of ANG-1 was investigated higher in SAP+BMSCs group than in normal, SAP, and SAP+anti-CXCR4 BMSCs groups. ((b), (c), and (e)) There was a significant higher expression of VEGF in SAP+BMSCs group than in normal, SAP, and SAP+anti-CXCR4 BMSCs groups at posttransplant days 1 and 4, with a subsequent decrease at day 7. ((f)–(h)) The mRNA level of HGF, TGF-β, and CD31 was significantly higher in SAP+BMSCs group than in normal, SAP, and SAP+anti-CXCR4 BMSCs groups. Data are expressed as mean ± SD (^# for sham versus NC, and for SAP+BMSCs versus NC, ^†, ^††, and ^††† for SAP+BMSCs versus SAP, , , and for SAP+BMSCs versus SAP+anti-CXCR4 BMSCs at each corresponding time point) (NC, normal control, ANG-1, angiopoietin-1, VEGF, vascular endothelial growth factor, SAP, severe acute pancreatitis, and BMSCs, bone marrow-derived mesenchymal stem cells).

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