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Stem Cells International
Volume 2015, Article ID 319238, 15 pages
Research Article

Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis

1Department of Molecular Cell Biology, Vilnius University, LT-08662 Vilnius, Lithuania
2Proteomic Center, Institute of Biochemistry, Vilnius University, LT-08662 Vilnius, Lithuania
3Clinics of Obstetrics and Gynecology, Faculty of Medicine, Vilnius University, M. K. Čiurlionio Gatvė 21, LT-03101 Vilnius, Lithuania

Received 15 May 2015; Revised 14 July 2015; Accepted 15 July 2015

Academic Editor: Peter J. Quesenberry

Copyright © 2015 Jurate Savickiene et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Proteome differences associated with myogenic, adipogenic, osteogenic, and neurogenic differentiation of late second-trimester AF-MSCs are presented in Supplementary Table 1 (see Supplementary Table 1 in Supplementary Material available online at For a comparison of the proteome profile, we presented a sample of normal cultured AF-MSCs from second-trimester (16 weeks, passage 5), cultured under appropriate conditions to induce myogenic, adipogenic, osteogenic, and neurogenic differentiation. The SYNAPT G2 High Definition Mass Spectrometry based analysis demonstrated 1423 proteins expressed in AF-MSCs. The relative protein expression ratios were calculated for each differentiation, and proteins with the expression ratio above 1.5 compared with undifferentiated control were selected and presented in Supplementary Table 1.

  1. Supplementary Material