Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro

Figure 4

Western blotting showed the upregulation of expression of Schwann cell markers and the downregulation of expression of neural crest markers except for SOX10. Expressions of Schwann cell markers including GFAP, CNPase, P75, and S100β by REMSCs and SC-like cells were shown in (a); expressions of neural crest markers including SOX10, nestin, vimentin, and CD44 by REMSCs and SC-like cells were shown in (b); the experiments were replicated three times, and β-actin was used as a loading control. Quantitation of each marker was calculated using morphometric analysis with ImageJ software. Each bar showed the ratio of the expression level of marker protein to β-actin (c, d). The data were presented as mean ± SEM of three independent experiments. represented significant differences when compared between RMSCs and SC-like cells.

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